Mycoremediation is a widely researched method using mycelia to clean bacterially contaminated water bodies, such as the Bothell Wetlands, which has been contaminated by crow roosts. This research aims to analyze and quantify the remediative properties of the fungus Stropharia rugosoannulata on the bacteria Escherichia coli, Salmonella enteritidis, Campylobacter jejuni and Klebsiella pneumoniae. Additionally, the remediation of the antibiotic resistance genes (ARG), Tet[A], Tet[B], Tet[M], StrA, StrB, blaCMY and blaCTX genes, was examined. One pound rye-seed bags were inoculated with liquid Stropharia. After three weeks, 35g of seeds were used to inoculate 60g of sterile wood chips in four 250mL bottles, for an incubation period of 3 weeks. Including a control bottle containing 60g of wood chips, we added 150mL of wetland water, spiked with 3500 CFU/100mL of the aforementioned bacteria, to each of the 5 bottles that were shaken via rocker. The following water retention times were chosen based on past experiments: 5min, 1hr, 5hrs, and 24hrs. When performing membrane filtration we used duplicate volume of 50ml for both genotypic analysis (qPCR), and for colony counting on selective plates. We analyzed the remediation using colony forming units and quantified using gene copy numbers (qPCR). The remediation of the following ARG was the highest after 1 hour of retention time: StrB (88.6%), Tet[M] (83.5%), and Sul-1 (23.2%), while blaCMY (100 %) and StrA (73.8%) had the greatest remediation after 24 hours. Results showed no remediation for Tet[A] and Tet[B] for any retention time. The remediation of Campylobacter (83.6%), Salmonella (97.6%) and Klebsiella (93.23%) was the highest after 1hr of retention time, while E.coli (27.61%) showed a greater remediation after 24hrs. The exponential increase in anthropogenic activities promoting bacterial contamination of the ecosystem and the spread of antibiotic resistant bacteria, highlights the urgency to find ways to mitigate them.