Individuals with ambivalent and avoidant adult attachment styles are more likely to use negative coping strategies such as denial and disengagement, while individuals with a secure adult attachment style are more likely to use positive coping strategies such as reappraisal and support seeking. Certain negative coping strategies are linked with mental health problems such as depression and anxiety. Most research regarding the relationship between coping strategies and adult attachment style has been done on undergraduates and veterans. No research to date has examined gender as a moderator of the relationship between adult attachment style and coping strategies. However, research suggests that both coping strategies and adult attachment styles vary by gender. In the present study, a series of multivariate regression analyses were conducted to determine whether gender moderated the relationship between close, and anxious adult attachment styles and emotional support seeking, and substance abuse coping mechanisms. Participants (N=385) completed self-report measures of coping strategies and adult attachment styles online. This project may reveal that gender changes the relationship between adult attachment style and coping strategies. This information may help inform psychological interventions for individuals with maladaptive coping strategies and improve our ability to predict who might engage in maladaptive coping strategies.

Ischemic preconditioning (IPC) is a robust, neuroprotective phenomenon in which a brief ischemic exposure confers resistance to injury from subsequent prolonged ischemia. Characterizing IPC may provide insight into better treatment options for those at high risk of ischemic stroke. Microglia, the immune cells of the brain, play an important role in the immune response to IPC. Previously, our laboratory found that the type 1 interferon signaling pathway in microglia is important in IPC-mediated neuroprotection. This signaling pathway is dependent upon activation of Toll-like receptor 4 (TLR4) and type 1 interferon receptor (IFNAR1). We hypothesize that in this pathway, damage-induced molecular patterns (DAMPs), which are released by brain tissues under ischemic conditions, activate TLR4 resulting in a signal cascade that activates IFNAR1, leading to phosphorylation of signal transducer and activator of transcription 1 (STAT1). Phosphorylated STAT1 (pSTAT1) then forms a complex with other proteins and induces transcription of multiple interferon-stimulated genes (ISGs). ISG expression alters the microglial phenotype, leading to neuronal and axonal protection against subsequent ischemia-related brain injury. The kinetics of type 1 interferon signaling in microglia are not yet fully understood. We aimed to further characterize this pathway by culturing primary microglia from wild-type mice, exposing them to TLR4 agonists or type 1 interferons directly, and quantifying pSTAT1 levels using flow cytometry at multiple time points. A time course of STAT1 phosphorylation in response to innate immune stimuli will provide a clearer picture of the kinetics of microglial type 1 interferon signaling in the setting of ischemia. These findings will enable us to optimize experimental timing for future experiments involving more complex and physiologic stimuli. Optimization of the kinetics of the pSTAT1 assay will also allow us to investigate how genetic ablation of specific innate immune signaling pathways (like TLR4 or IFNAR1) might modulate the microglial response to ischemia.

Nearly 22 million HIV-positive people are receiving antiretroviral therapy in order to suppress their HIV infections. They need consistent viral load monitoring to track viral suppression and detect the possibility of viral rebound. Nucleic acid amplification tests (NAATs) are used to measure the viral load in a patient's blood. Traditional, laboratory-based NAATs require complex robotic systems to automate HIV RNA purification, amplification, and detection from blood. Since the majority of those living with HIV are located in low and middle income countries, there is a need for rapid viral load monitoring at the point of care (POC). We aim to provide accessible HIV viral load testing through low-cost, integrated POC NAAT devices. These proof-of-concept devices operate as a two-step assay to extract and detect nucliec acids in blood. An electrophoretic separation technique called isotachophoresis (ITP) separates HIV RNA from other components in a blood sample. An isothermal nucleic acid amplification assay amplifies the purified, concentrated nucleic acids in order to detect and quantify their presence. We present our development of a novel ITP system to remove potent contaminants from Proteinase K (PK) digested serum and extract highly pure nucleic acids automatically. Through computational modelling, a dual trailing electrolyte (TE) buffer system was designed to exploit the isoelectric point of PK for its removal, while simultaneously concentrating nucleic acids away from serum components. We demonstrate system control through comparison of experimental observations to model predictions by performing dual-TE ITP on pH paper. We also show that the dual-TE system improves upon previous limits of detection for DNA extraction and detection from complex samples. Our system processes 40 microliters of blood in 20 minutes using only simple buffers, a paper strip and an electric field - making it an ideal tool for use in a rapid NAAT for HIV viral load testing. 

Secondary metabolite chemicals are specialized chemicals produced by plants that serve specific roles in plant survival beyond aiding in growth or development. Sometimes, the presence of these chemicals negatively impacts surrounding species, a phenomenon known as allelopathy; an example of this is the inhibition of germination of neighboring species. One common non-native species that is known to have high concentrations of leaf secondary metabolite chemicals is Plantago lanceolata (ribwort plantain). Focusing specifically on prairies ecosystem implications, this experiment aims to test the allelopathic effects of Plantago leaf secondary chemicals on the germination of native prairie species. The predicted outcome of this experiment was that increased concentrations of Plantago extract would lead to decreases in germination quantity. To accomplish this, numerous extraction concentrations (including a controlled no-extract treatment) of Plantago leaf chemicals was applied to several prairie species. These species were additionally tested in the presence of high concentrations of yarrow and lettuce extracts; yarrow is another species that contains high concentrations of secondary chemicals, while lettuce leaf material lacks substantial secondary metabolites. Prairie seeds germinated in the presence of secondary chemical extracts include yarrow, Oregon sunshine, Roemer's Fescue, Blue wildrye, and Plantago. 1,400 seeds per species were placed in petri dishes and germinated in either spring or summer growth chambers based on each species' germination requirement. Germination data were analyzed to determine the significance of germination inhibition by each leaf extract. Because Plantago is a non-native and potentially invasive species, it is important to understand the potential for native ecosystem disruption. Additionally, since yarrow and Plantago seeds were germinated in the presence of extracts from their own species, we could determine whether extracts have stronger effects on disparate species than on the species that the extract is derived from. 

Recurring slope lineae (RSL) observed on Mars appear to be flows of liquid water because they seasonally propagate down sunny slopes in the spring and fade during the winter. Liquid water suggests the potential for life on Mars’ surface and has implications for future exploration; however, recent hypotheses contend that RSL form via dry sand avalanches. To test wet vs. dry flow hypotheses, we analyzed images and topographic data from Garni crater taken by the High Resolution Imaging Science Experiment (HiRISE) during the Martian summer and winter. Our results show that RSL size correlates with areas where we expect high windblown sand deposition, such as on the SE lee side of the crater, or in small gullies below large headwalls. Additionally, RSL do not appear on the wind-scoured NW side of the crater. These surface relationships indicate that RSL in Garni crater form via a dry process in which windblown sand is deposited by prevailing NE winds and seasonally avalanches down steep slopes. To further test our conclusions, we are examining additional confirmed RSL sites on Mars to see if they display the same behavior.

Seasonal dark streaks on Mars known as Recurring Slope Lineae (RSL) propagate down steep, warm slopes and appear to be liquid water flows. However, the mechanism behind RSL formation is controversial, and both dry granular flow and percolating water hypotheses have been proposed. To determine if water is responsible for RSL formation, I investigated similar dark streaks generated by percolating water in the McMurdo Dry Valleys (MDV) of Antarctica, an extremely cold and dry Mars analog site. The goal of this research is to identify the source of water to the MDV streaks, and to compare the MDV streaks to Martian RSL. I characterized the MDV streaks by (1) analyzing a ~30-day time-lapse video of the streaks collected on site, (2) investigating the drainage hydrology, and (3) comparing hundreds of satellite images of the streaks from 2003-2017. My results show that the MDV streaks are very different from Martian RSL, which indicates that water is not involved in RSL formation. Unlike Martian RSL, I found that MDV streaks propagate downslope at much slower rates compared to Martian RSL, and do not grow and retreat seasonally. Furthermore, MDV streaks have distinctive patterns not apparent in Martian RSLs, and rapidly darken/lighten in response to relative humidity changes. Finally, MDV streaks form in response to extremely warm summer temperatures, conditions which are unlikely to prevail on Mars. These results are important for understanding how and if liquid water occurs on Mars’ surface.

Fragile race avoiding is defined as the tendency to refrain from talking about race or racial issues to hide one’s bias due to sensitivity concerning the topic. Some even demonstrate discomfort or incoherence when directly talking about racial issues. This is common amongst whites who want to avoid conflict regarding racism. Although white people may believe that avoiding racial topics stops disagreements from arising, fragile race avoiding can promote ignorance about stereotypes and inhibit cultural awareness. We believe that this is a method in which individuals suppress their discomfort towards blacks. Therefore, we predict that a higher score on fragile race avoiding will correlate with higher levels in overall racism. We tested this prediction in a study in which UW undergraduate students talked about various scenarios with a research confederate to prompt a discussion about current day racial issues. Coders watched these taped interactions and rated various categories of microaggressions on a scale from zero to three. Scores of zero for fragile race avoiding indicates that the research participant is comfortable talking about racial issues and brings up race without being prompted by the confederate. Meanwhile scores of three indicate that the participant actively avoids mentioning race and shows palpable discomfort throughout the interaction. For the scores on overall racism, coders considered the interaction as a whole and determined how black individuals would feel when in a room with the participant. A score of zero represents comfort and understanding of the racial implications these topics were created to induce, while a three shows that the participant’s racist beliefs are explicit and clear. We hypothesize that there will be a positive correlation between the scores on fragile race avoiding and overall racism, indicating that fragile race avoiding is a modern form of racism in which individuals suppress their discomfort among blacks.