Research has shown that wildfire smoke exposure is associated with various respiratory health effects. To help protect the lungs, respirator masks that are labeled as N95 can be worn to filter out fine particulate matter produced from wildfire smoke. Previous studies show that video-based education is effective in increasing knowledge and intervention practices among viewers, and it was hypothesized that an N95 mask instructional video could help educate the lay-public. A video-content needs assessment was conducted using semi-structured key informant interviews. Eight experts knowledgeable in air quality, message communication, N95 respirators, and respiratory and cardiovascular health were interviewed for important content needs. Response themes were identified and used to inform the content and style of the video entitled, Smoke from Fires: N-95 Respirator Masks. A Knowledge, Attitude, and Practice (KAP) survey, using a 5-point likert scale, was created to assess the video’s knowledge transference, and effect on attitudes and commitment to practice use. The KAP survey was given to undergraduate students before and after viewing the video. The results from our key informant interviews included instruction on proper use, mask limitations, incorrect alternatives, and appropriate use of language, images, and tone. The KAP survey results indicate the video enhanced the participants’ knowledge of N95 masks with regards to proper fit, incorrect alternatives, and limitations. Additionally, attitudes of increased ease of use and mask effectiveness were demonstrated. Since this study investigated a video-based intervention, possible future research includes testing the effectiveness of different education methods on N95 mask fit, in addition to knowledge, attitudes, and commitment to practice.