Living cells must go through several phases as they grow and divide. During the phase known as mitosis, attachment of microtubules to chromosomes through specialized protein structures called kinetochores is indispensable for chromosome segregation. The process is prone to error due to the random nature of chromosome-microtubule attachments. A protein located near kinetochores, Aurora B kinase, phosphorylates erroneous connections, facilitating detachment of the microtubules and allowing another opportunity for correct attachments to form. Classic studies have suggested that a lack of tension on kinetochores is an error indicator for cell division checkpoints, because artificially applying force to chromosomes was sufficient to prevent their reorientation from mal-oriented configurations in insect spermatocytes. Based on this observation, a popular belief is that tension suppresses Aurora B triggered detachments. However, the relationship between chromosome reorientations observed in insect spermatocytes and the activity of Aurora B remains uncertain. To test the involvement of Aurora B, we utilized a small molecular inhibitor, AZD, to specifically reduce the activity of Aurora B. House cricket’s spermatocytes were extracted and soaked in a mixture of Grace’s insect medium and AZD to allow the inhibitor to permeate the cell membrane. After AZD treatment, the cells were spread on a chamber slide and filmed under a phase contrast microscope for approximately 5 hours. Our results show near complete prevention of cell division in the AZD treatment group. In the control group without AZD, transitions from metaphase to anaphase were much more frequent. Thus, Aurora B appears to have a crucial role during insect spermatocyte mitosis. This observation brings us one step closer to a definitive test of whether the tension-suppressed reorientation of chromosomes in insect spermatocytes is dependent on Aurora activity.