Chlorophyll concentration is an indicator of plant health, but is challenging to quantify. As a result, normalized difference vegetation index (NDVI) imaging was developed to help solve this problem by making use of of chlorophyll’s varying reflectance of light waves. This is a proven and powerful tool for observing terrestrial vegetation, allowing for efficient monitoring of living plant populations worldwide. However, no research had been published regarding the use of NDVI for submerged aquatic vegetation, thus our team sought to apply the technique to an aquatic environment, specifically seagrass beds in Queensland, Australia. Seagrass provides both nutrition and habitat for marine life, as well as carbon dioxide storage. The ability to monitor seagrass ecosystems is essential to understand and, in turn, preserve them. We saw NDVI imaging as a potential improvement to current seagrass monitoring methods and conducted experiments to find what issues this technique has in the underwater environment. After testing many aspects of NDVI imaging on in-situ seagrass beds and other test environments, we ultimately found it was not a viable option. This is largely due to basic optical transmission characteristics of water.