Mussels, Family Mytilidae, are vital to intertidal ecosystems and to a burgeoning aquaculture industry. They act as ecosystem engineers within their environment by controlling resource availability to other organisms and by shaping the structure of their habitat. Unfortunately, with the rise of anthropogenic climate change, mussel populations are seeing growing threats to their ability to survive and reproduce. This includes lower recruitment, increased susceptibility to disease, and weakening of the byssal thread that adheres mussels to their substrate. Understanding the life history of these organisms, in addition to what food sources and conditions are most suitable for their growth, is essential for preserving this economically and ecologically vital species group. Mytilus trossulus, a common species of saltwater mussel ubiquitous in the aquaculture industry was used in this study. The mussels were grown at two different depths on aquaculture lines for comparison of the environmental effects at those depths. The first layer at 1 meters depth consisted of a mixed surface layer rich in phytoplankton growth and represented a warmer, more variable ocean environment. The second layer was found below the surface mixed layer at 7 meters depth and represented a colder, more constant environmental regime. By analyzing the food sources of these mussels through fatty acid extraction, we will draw conclusions not only about what nutrients are most beneficial to mussel growth, but also what effects the environmental differences between the two depth strata has on the mussels. The purpose of this study is to both provide data for the care of mussels in aquaculture farms and to make inferences about what a changing climate will mean for these organisms.