Zebrafish are widely used for the regenerative capacity of their fins and its application to human bone growth and remodeling. The purpose of this experiment is to investigate the role of neurotransmitter systems in the process of fin regeneration and their impacts on de-differentiation of bone cells proximal to the amputation plane. Neuronal signaling is vital to all processes in the human body, however a link between specific neurotransmitter systems and the formation of bone has yet to be fully determined. From our past experiments we have identified a link between botulinum toxin (BTx), a common neurotoxin that inhibits the acetylcholine neurotransmitter system, and Nicotinamide adenine dinucleotide (NADH) levels in the fin during regeneration. NADH levels are indicative of cellular metabolism and are increased in cells that are actively differentiating into mature osteocytes. From this finding we have expanded our study to two neurotransmitter systems, the cholinergic, which focuses on acetylcholine, and the adrenergic, which focuses on epinephrine. From large ligand libraries we formed a ranking of compounds based on their application to bone and prior use in vivo. The four initial compounds utilized were propranolol, galantamine, ICI 118 551, and hemicholinium. Zebrafish were subjected to fin amputations followed by seven days of dosing. Following this procedure, the levels of NADH, green fluorescent protein (GFP), and bone mineralization were analyzed using fluorescent microscopy. GFP is a protein used as a reporter of gene expression. Each condition showed a variation in at least one of these criteria. Three conditions showed an initial increase in NADH, similar to the results of BTx. These results suggest that the relationship between neurotransmitter signaling and regeneration may be composed of many complex interactions between signaling molecules and pathways, and we will continue to investigate this as the experiment progresses into the remaining neurotransmitter systems.