Disorders and diseases of chronic pain have placed a tremendous burden on modern society, imposing myriad consequences on many individuals and populations. Despite extensive investment to date, therapies to treat chronic and acute pain remain limited; the most-widely used, researched, and prescribed drugs often act generally upon the nervous system. This characteristic of most existing therapies carries with it many unintended consequences and unwanted effects for pain patients. The limitations of these therapies are often traceable to our limited understanding of the neuronal circuitry responsible for the sensation and transduction of noxious or other pain-inducing stimuli. We utilize zebrafish in our forward genetic screen to identify mutations that disrupt the ability to perceive pain. Behavioral tracking of larval zebrafish, specifically the progeny of mutated genetic lines, allows us to screen for a large number of potential phenotypic mutations. By identifying mutant lineages in this screen, we hope to further elucidate and characterize the neuron cell populations responsible for nociception. Our genetic screen has identified several lineage of fish that lacked the behavioral “escape” response typical of embryonic zebrafish when immersed in a noxious chemical environment, in this case, 10µM allyl isothiocyanate (AITC), the pungent compound in mustard oil. Subsequent complementation to known mutants suggested that one of these families carried a mutation in a previously identified nociceptive ion channel, TRPA1, which is specifically expressed in somatosensory neurons, and has been shown to be required for the pain response induced by noxious chemicals, including AITC. We have crossed mutants into well-characterized genetic backgrounds and used RNA-seq based mapping to narrow these lesions to a region of Linkage Group 9. Following fine-mapping, we will subsequently study the role these gene(s) play in the sensation of pain. Our findings suggest that our screening strategy is likely to be successful and is feasible for identifying genes involved in nociception.