Honeybees (Apis mellifera) use a caste system to organize individuals into different roles. The queen is the only bee in the hive to ingest royal jelly and the only fertile female. Many sterile workers perform different jobs in their short lives, moving from larvae-nurse to keeping the hive clean to foraging for food. At each stage the worker bees learn a new task. Such different phenotypes and behavior would indicate a difference in gene expression in the brain. In a population of organisms that are genetically similar, these changes in gene expression must be explained in chemical modifications of the genome, or the epigenome. Thus far, much attention has been given to DNA methylation as an epigenetic feature known to be associated with variation in gene expression. Royal jelly, however, contains butyrate, a histone deacetylase inhibitor, which suggests that histone tail modifications could be a major mechanism for regulating gene expression in queen bees. To further investigate the role of histone modification in determining honeybee caste division, we are performing chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-seq) to profile key chromatin modifications: H3K27ac, and H3K27me3. H3K27ac and H3K27me3 are histone modifications that have opposing effects; where H3K27ac marks active regions of the genome, and H3K27me3 is indicative of repressive chromatin. We anticipate when using ChIP-seq to map chromatin modification in the brains of queen and worker bees, we will observe key differences in genome regulation and be able to identify genes important to honeybee behavior. As an initial step toward achieving our goal, we describe the chromatin state of brains from forager honeybees.