Biomarkers capable of diagnosing and tracking the progression of Parkinson’s disease (PD) are urgently needed. Saliva has been explored as an alternative to a more traditional sample source such as cerebral spinal fluid (CSF), which involves painful and invasive collection processes, and blood. Indeed, PD patients typically have dysfunction of salivary production, indicating that the disease involves this organ system. In order to increase sample availability, many protocols use cotton or plastic to stimulate saliva production. However, this raises the question of how these materials affect salivary protein composition, specifically in regards to proteins of interest as biomarkers. In order to explore these effects I incubated various materials potentially capable of stimulating increased saliva production in saliva samples and measured the concentration of alpha-synuclein (α-syn), a protein believed to be important in PD pathogenesis and a possible biomarker, and total protein. By comparing the concentrations of α-syn, total protein, and the α-syn:total protein ratio between the various material groups and control (no material) groups I am able to determine whether the materials increased, decreased, or had no effect on protein concentration. It was hypothesized that all tested materials would decrease both α-syn and total protein concentrations by some degree due to protein attraction to the materials. The results of this study contribute to a clearer basis for salivary biomarker studies involving induction techniques, facilitating collection techniques in a clinical setting, particularly in older patients with decreased saliva production.