This exploratory study investigates levels of stress among informal caregivers of cancer patients and the amount and kinds of social support they received while providing care. Previous research indicates that informal caregivers, defined here as family members or friends providing care to loved ones, often times experience extended periods of stress that can be reduced with increased social support. Social support includes the instrumental, informational, appraisal, and emotional forms of assistance caregivers provide. The aim of this study is twofold: to examine why previous social support services at a care facility in the Pacific Northwest had fluctuating participation rates, and to find strategies to strengthen social support for caregivers through understanding the levels of stress and support they experienced. I hypothesize that fluctuation was due to a lack of knowledge of support services and receiving support outside of the agency. I use a mixed methods design with a quantitative survey and a qualitative focus group component. Participants are given surveys inquiring information about the levels of stress they experience in their daily lives and the amount of support received from others. The focus group consists of guiding questions including open-ended questions about their experiences with caregiving, levels of stress, coping methods, forms of social support, observations on health changes, and perceptions of supportive services. I expect to find that caregivers receiving social support from family and friends will report experiencing less stress compared to caregivers that primarily receive social support from healthcare professionals. Overall, this study provides a deeper understanding of the extent to which caregivers feel social support and highlights barriers to social support service use at an organizational level. These findings have implications for existing knowledge on caregiver support and increases the knowledge base of stress and support of informal caregivers.

All organisms require the pyridine nucleotides NAD(P)(H) to perform various cellular functions, but their role in methylotrophy is not well understood. Methylotrophic organisms are able to use organic substrates without carbon-carbon as a sole source of energy and carbon. In metabolism, pyridine nucleotides provide reducing power for the cell to perform redox reactions in catabolic and anabolic processes. In contrast to energy-limited modes of growth, methylotrophy is predicted to be reducing power-limited as the oxidation of methanol or methylamine is coupled to phosphorylation of ATP and energy production. Furthermore, internal concentrations of NAD(P)(H) are predicted to be saturating so the balance between catabolic and anabolic charge ratios may be of greater importance than the relative concentrations. Consistent with these predictions, fluctuations in NADPH levels are observed during as an immediate response when the facultative methylotroph Methylobacterium extorquens AM1 is transitioned from multi- to single-carbon substrates. Known mechanisms to recycle pyridine nucleotides include the decoupling of NAD(P)H using NAD(P) kinases/phosphatases or transhydrogenases and the use of enzymes with dual specificity or isoenzymes. We propose a transhydrogenation cycle between the EMC and PHB pathway to be an important mechanism to maintain redox homeostasis in M. extorquens AM1. To demonstrate this, I will use select OMIC approaches to analyze wild-type strains during succinate to methanol transitions and compare wild type to an mtdB mutant, a strain without a functional methylene-H₄MPT dehydrogenase B (MtdB). In this mutant, the assimilatory blocks on methanol and methylamine are presumably due to the disruption of a key pyridine-nucleotide producing step. These results will provide increased understanding of the mechanisms used to balance production with consumption of redox equivalents and re-distribute carbon flux in M. extorquens AM1. Insight from this work has implications for engineering methylotrophs for biotechnological applications including the production of biofuels and value-added chemicals.

In cultures where children have regular contact with same aged peers during the first years of life, peer sociability plays a fundamental role at early stages. Currently, the only social emotional skills children are exposed to are those that they learn alongside academic learning. Additional social emotional skills are often not taught. These skills include social awareness, self-awareness, dealing with ambiguous situations, adapting to social situations, managing stress, and learning how to learn (Lindsay, 2013). It is especially challenging for English language learners (ELL), who have the additional task of learning multiple ways of communication, including both language and cultural rules. Research on social emotional learning has shown that when children are able to deal with, manage, and maneuver the social and emotional landscapes of their lives, their capacities to learn and function socially on all levels improve (Lindsay, 2013). This study seeks to compare the social emotional outcomes for ELL students with their native English-speaking peers in classroom settings. The study includes literature review and data analysis on data collected by Second Step Study researchers at the College of Education from approximately 5,000 children across 40 schools in 5 districts. One of the teacher-reported measurements used in the study is the Devereux Student Strengths Assessment (DESSA), a behavioral rating scale that measures skills associated with social-emotional competence, resilience, and academic success. The other teacher-reported measurement is the Strengths and Difficulties Questionnaire (SDQ), a behavioral screening questionnaire which measures children’s strengths and difficulties related to behavioral characteristics. Results from the measures will be compared between native English speakers and ELL students. The aim of this study is to increase teachers’ awareness of the importance of social emotional development of English language learners.

Currently, there is a huge waste of natural gas being vented or flared off all over the world resulting in large amounts of lost energy while the demand for liquid fuel continues to rise. This loss of energy can be reduced through the use of methanotrophs to converts natural gas to liquid fuel, also known as biological gas-to-liquid fuel conversion. This project is broken down into three areas: Strain Improvement, Process Optimization, and Recovery. My portion of the project is to help gather base-line fermentation data for the purpose of strain improvement. The ultimate goal of this project is to develop efficient fermentation process for the production of high lipid biomass. The methanotrophic bacteria strain is Methylomicrobium buryatense (5GB1). Each fermentation run is done in a 1L bioreactor (fermentor). Different mixed gases are used for each fermentation run. Optical density is used to monitor bacterial growth, and the produced gas is analyzed using a Gas Chromotography System. The different conditions tested are oxygen limited, methane limited, and methanol with a control set up of unlimited growth. Unlimited growth is found to have the fastest growth rate with a doubling time of 3 hours. Oxygen limited and lethane limited have a slower growth rate when compared to unlimited growth, which follows the expected bacteria behavior under limiting conditions. Methanol causes the bacteria to go through a longer phase of lag time and has a slower doubling time compared to unlimited growth as well. After testing the growth rates under these conditions, the produced biomass is tested for the amount of lipids in the cells. The final results are then compared to find the optimal intersection between lipid biomass production and bacteria growth rate.

Macrophages of our immune system can be broadly classified as pro-inflammatory M1 or anti-inflammatory M2 macrophages. Imbalance in the ratio of these two phenotypes has been associated with various chronic diseases, suggesting that targeting drugs to specific populations of macrophages may achieve therapeutic benefit. Phage display is one method of identifying peptides that can be used for targeting cell populations. In whole-cell peptide phage display, a library of bacteriophage, in which individual phage display a unique peptide sequence on a coat protein, is applied to target cells. Phages that preferentially bind target cells are isolated, and their peptide sequences can then be obtained by DNA sequencing. Targeting M1 macrophages has possible applications in treating arthritis, diabetes, and atherosclerosis. Recently, we identified a novel phage-bound peptide sequence (hM1pep), using phage display, that exhibited selective binding to M1 macrophages. I synthesized the peptide form of hM1pep and evaluated its selectivity of M1 macrophage binding in vitro using flow cytometry; however hM1pep showed insignificant binding. Tumor-associated macrophages (TAMs), a type of M2 macrophage, have been shown to facilitate tumor growth and are associated with decreased survival of cancer patients. Previously, we identified a peptide that preferentially bound to TAMs in mice. We demonstrated that peptide-mediated TAM elimination improved survival in mice, suggesting that an analogous strategy in humans could be a potential cancer therapy adjuvant. In this work, I attempted to identify human M1 and M2 macrophage binding peptides by panning against human M1 and M2 macrophages, while using M2 and M1 macrophages as a subtractive panning step, respectively. Targeting M2 macrophages has potential uses in treating diseases associated with increased tissue levels of M2 macrophages, including cancer, fibrosis, and asthma.

Currently there is extensive interest in the health of honeybee colonies, both in the United States, and worldwide. A healthy and functioning agricultural system requires honeybees, and bees in turn, require nutritious, diverse forage that includes both nectar and pollen. This study involved analyzing the pollen collected by honeybee colonies maintained on the campus of Edmonds Community College, and matching that with pollen collected directly from local plants. We isolated pollen from honey collected and stored by the bees and also collected pollen directly from cells in the hive. In order to identify the different pollen species, the pollen was chemically extracted and acetolyzed to remove sugars and waxes. The treated pollen was then stained, slide-mounted and viewed by light microscopy. These pollen samples were compared to samples of pollen that were collected directly from local plants as well as to existing pollen databases. Preliminary analysis revealed the presence of pollen from brassica, salvia, and trifolium. We were unable to identify some of the extracted pollen species. During the coming foraging season we will expand our database of locally-collected pollen and will generate a local pollen identification key. This will allow us to conclusively identify all of the pollen found in the hives. Results of this study will help us track what the bees are eating and will inform decisions about what best to plant to insure healthy colonies.

Previous research on young adults shows a negative relationship in college-aged students between alcohol-related behaviors and religiosity, giving evidence that religiosity acts as a protective barrier. Verifying that religiosity acts as protective behavior allows for a clearer understanding of possible strong deterrents towards risky alcohol-related behavior. To date no study has investigated religiosity and alcohol use in both the U.S. and Sweden, an international sample that is predominantly Christian and non-religious. The present analyses aims to compare the U.S. and Sweden in religious affiliation and alcohol use. Participants completed a baseline online survey as part of a larger trial that assessed risk behavior in adolescents. In the present analyses, the severity of alcohol use was measured with the 10-item Alcohol Use Disorders Identification Test, alcohol related negative consequences was measured with the 18-item Young Adult Alcohol Problems Screening Test, and frequency of religious practices was measured with one-item. We predict a negative relationship between alcohol use and alcohol related negative consequences and religion. Regression analyses were run assessing the contribution religious affiliation has on alcohol use related problems. Analyses revealed a significant main effect of religious affiliation on AUDIT and YAAPST, such that religious affiliation was related to lower alcohol use severity and related negative consequences. This further establishes religiosity as a possible protective measure to alcohol related behaviors. Thus, religious affiliation may prevent alcohol use in adolescents. One limitation is how narrowly we tested religiosity. Future research might include investigation of religious groups such as “youth groups” or religious service attendance and alcohol use to activities holding similar properties to religious groups and behaviors.

College student drinking continues to be a concern due to high consumption rates and negative consequences associated with excess use. Both male and female students are at risk for heavy alcohol use and related consequences. Symptoms of depression are also reported frequently amongst college students, particularly female students who tend to be at higher risk than males for depression. Evidence suggests that depressive symptoms are often correlated with increased alcohol use. Alcohol consumption has also been related to perceived social norms of peer drinking, i.e. the perceived amount of drinking that other students and peers typically engage in. Injunctive norms, which refer to perceived approval or disapproval of a behavior by other students, may be highly regarded by students as important for decisions regarding their own behavior. Furthermore, it is hypothesized that this relationship may shift with an increase in depressive symptomology in females. Injunctive norms-indicating approval from others-have been linked to increased alcohol use in this population. Prior research also suggests that female drinking tends to be more strongly correlated with typical same-sex student alcohol injunctive norms more than their male counterparts. Thus the proposed study examines the moderating role of depressive symptoms and gender on the relationship between injunctive norms and alcohol use in college-age students. The goal of this study is to further understand the nature of the relationship between injunctive norms and alcohol use, and whether depressive symptoms enhance risk from injunctive norms in females more than males.