Recent longitudinal studies show a trend of declining emotional health paired with an increased demand for counseling services among college students. It has been proposed that this population faces difficulties in regulating emotions, especially in the face of challenges that are new and unique to them. In the clinical field, mindfulness practice is gaining evidence as a way to increase individuals' self-awareness, acceptance and present moment attention – qualities that have robust evidence of being beneficial for the effective regulation of emotions. The rationale of this research is that mindfulness practice could potentially increase the capacity for allowing painful emotional experiences and thus reduce maladaptive automatic responses to stressors (i.e. denying or suppressing negative emotions). The main aim of this study is to explore whether Dialectical Behavior Therapy (DBT) mindfulness skills, which are six distinct skills designed to cultivate the different components of mindfulness in everyday life, can be efficacious at reducing difficulties in emotion regulation and increasing mindfulness in a non-clinical population of college students. In addition, since few studies have evaluated the feasibility and acceptability of mindfulness-based interventions in college settings, this study fills in the gap by evaluating feasibility and acceptability using specified, testable criteria, and by exploring an alternative method of instruction: using audio recordings of DBT mindfulness practice. The study design is a between-subjects randomized controlled trial comparing an intervention group which will receive a two-week mindfulness training, with a non-intervention group which will not receive the intervention. The hypotheses of this study are that the intervention will be 1) efficacious at increasing mindfulness, 2) reducing anxiety and 3) decreasing emotion dysregulation, will be 4) acceptable in terms of participant retention and opinion toward the intervention, and 5) feasible in terms of adequate participant recruitment and compliance to the intervention protocol.

 I will argue that youth homelessness and society are intertwined existences and that we should move beyond narratives of blame and silence in order to find a more unconditional compassion for homeless youths. I will draw from my experiences volunteering at ROOTS Young Adult Shelter over the past three and one-half years, as well as from interviews conducted there during Summer 2013. The material gathered with be analyzed through Timothy Pachirat's (2013) "politics of invisibility" and Donna Haraway's (2007) "becoming with." The narrative we use to structure our opinions of homeless youths, what I call the personal agency narrative, attributes their situations to their laziness, addictiveness, and/or generally disagreeable behavior. However, an examination of the encounter with the homeless youth, which informs the personal agency narrative, reveals that the economic, political, and cultural contexts of youth homelessness, as well as the personal experiences (before and after becoming homeless) of homeless youths are left invisible. This, I believe, is a microcosmic analog to the broader pattern of dealing with youth homelessness as a society, within which we find a conditional compassion--we only are able to empathize with certain homeless youths-- which is tied to the personal agency narrative. I will argue that we should move beyond this conditionality, recognizing the multiple ties that bind society to youth homelessness, and vice versa, to see that society is in a state of becoming with youth homelessness. We therefore have a responsibility to youth homelessness.

The 21st century has seen the emergence and re-emergence of novel infectious diseases. Following in the footsteps of its communicable predecessors, the recent emergence in Saudi Arabia of a SARS-like novel coronavirus, or Middle East respiratory syndrome (MERS-CoV), since 2012 has weaved narratives of globally interdependent economic, political, and social intimacies. I will examine the contrasting narratives that reflect investigations and responses to this novel threat from Middle Eastern, European, and North American media networks, the WHO, the global biomedical community, and the Saudi Arabian Ministry of Health through several intersecting discourses: intellectual property rights and viral sovereignty; the deliberation on the taxonomy of the novel coronavirus; and geopolitical differences in information sharing and biosecurity in the context of emergent 21st century epidemics. Using Priscilla Wald’s concept of outbreak narratives and the imaginative hold of myths as a framework, I will explore how the stories on the global effort to investigate and respond to the MERS outbreaks continue to be revealed, repackaged, and transformed as experts around the world scramble to uncover its epidemiological pathways. I argue that the consideration of these counter-narratives are critical for rethinking underlying, potentially harmful, factors beyond dominant epidemiological and biomedical discourses surrounding the MERS epidemics. Such considerations may serve as a microcosm for biopolitical barriers in the response to past, present, and future emergent diseases. These counter-narratives should be regarded in light of uncertainties that still cloud the etiology and transmission of the virus which has since infected 243 people and claimed 93 lives in 11 countries in Europe, the Middle East, and Southeast Asia.

 In 2009, the World Health Organization declared swine flu to be the first global flu pandemic in 40 years as a result of the outbreak of cases in Mexico. Although the mortality rate of the disease was lower than predicted, the narrative constructed around the disease offers important takeaways regarding how an outbreak is perceived and what groups become associated as disease carriers. Incorporating knowledge of Mexican-American relations, past and present, in the analysis of the media coverage broadens the understanding regarding the roles that racism and xenophobia play in responses to swine flu. However, the U.S media’s blaming of Mexico, especially when connecting to issues of immigration and borders, draw attention away from American culpability in the H1N1 experience. Using my digital medium as a holding space and synthesis for different thought provoking articles, imagery, and videos about this subject will allow the audience to understand the systematic conditions that influence the spread of diseases like H1N1. For example, by unpacking the capitalist economic imperatives of NAFTA (North American Free Trade Agreement), a policy signed off by Canada, the US, and Mexico to create the world's largest free trade area, I examine how its neoliberal policies encouraged transnational corporations to open up industrial pig factories and its impacts on the environment, human health, labor practices, animal welfare, and immigration. I argue that these factors are symptoms of a “NAFTA Flu”, which shaped unequal disease development conditions and unequal access to treatment in Mexico, a perspective that anti-Mexican media ignore. Therefore, critiquing the U.S media framings of the outbreak undermines the stigmatization and blaming of the specific populations in traditional outbreak narratives.

The purpose of this research is to investigate hidden health narratives on campus regarding the use of tobacco products to contextualize the legitimacy of smoke-free and tobacco-free university initiatives. This academic endeavor will also reveal the structural and institutionalized violence of the tobacco industry which targets marginalized communities to entice the uptake of regular smoking habits. An analysis of biopolitical power, structural violence, and social theory will help to reveal the hidden narratives of these populations, twice-targeted by the social stigmatization of tobacco use and the manipulative advertising of the tobacco industry. By exposing, analyzing, and deconstructing patterns of profit-driven tobacco marketing directed at student demographics, it is possible to examine geographies of blame regarding tobacco use, impact of tobacco use on non-smoking populations, and the misleading agendas behind the dominant narratives of tobacco industry advertising. By identifying and bringing to light these messages, it is possible to legitimize the anti-smoking and anti-tobacco movements happening at the University of Washington, and U.S. universities at large, with a critical analysis of the health risks involved.

When animals (humans among them) mourn, they honor the emotional bonds that make social living both desirable and possible.  Ebola, one of the most virulent primate diseases, wreaks havoc within this sociality, bringing death and triggering isolation.  Here I examine spaces of mourning - instances when mourning does or might occur - in the context of Ebola to explore how we can protect social connection in the face of disease.  Previous Ebola narratives have often portrayed mourning as an obstacle to public health.  Journalists and academics have largely ignored how isolating people from dying loved ones and depriving families of traditional mourning rituals affects the grieving process or how forcing people to quell grief as they exterminate Ebola-infected lab monkeys affects humans.  Only recently have scholars begun to explore difficulties Ebola survivors face grappling with grief amid stigma-induced isolation.  Here, I explore what it means to reread outbreak narratives in light of theories of mourning.  Drawing upon Judith Butler's concepts of grievability and precarious life, I challenge narratives touting extermination as the sole means of stopping Ebola from crossing species.  Together, these accounts highlight the shared vulnerability and capacity for social suffering uniting humans with other primates.  Through this work I explore how recognizing the significance of relationships within and between species vulnerable to Ebola might change human responses to this disease.  If we embrace this approach for Ebola, it opens new possibilities for handling other infectious diseases and for deciding which lives (among individuals of all species) count as grievable.

How was U.S. immigration policy shaped by the 2009 Swine Flu outbreak? My project will explore the relationship between immigration policy, xenophobia, and Swine Flu. The uproar in the US stemming from the 2009 Swine Flu (H1N1) outbreak revealed a racialized narrative that targeted and stigmatized Mexican immigrants. Rising xenophobic sentiments were exploited to further the cause of anti-immigrant groups and to justify immigration limits. For example, xenophobia was so prominent that some citizens blindly advocated for the closing of the U.S. - Mexican border without considering the economic effect of such an action. As part of this project I will explore xenophobia itself as an outbreak linked to the Swine Flu outbreak. How did this xenophobia outbreak infiltrate the U.S.? Were U.S. immigration policy makers susceptible to xenophobia? Were they “infected” by the xenophobic outbreak? Is xenophobic sentiment evident in immigration policy? I will draw on empirical data (like immigration statistics and public opinion polls), analyze rhetoric used in immigration debates, and explore media representations, to reveal the connections between Swine Flu and U.S. Immigration policy. I argue that Swine Flu, and the xenophobic discourses surrounding it, had a profound effect on contemporary immigration narratives.

This paper analyzes the juxtaposing narratives between various documents and media portrayals from mining corporations, the Peruvian government, and the affected indigenous populations, focusing on how many of the more dominant narratives can often be misleading in reflecting the serious health effects from mining. Peru’s mining industry significantly influences the health of surrounding indigenous communities due to high levels of air and water pollution. Unfortunately, there is not a great deal of sincere concern for the wellbeing of these communities from either the corporations exploiting their land or the Peruvian government. Despite their efforts to express their suffering through protests, indigenous communities have seen few results. The mining industry and its detrimental health effects are often overlooked, and therefore many of these dominant corporate narratives and our own capitalist desires are all that we have to shape our understanding of how mining industries operate in other countries, such as Peru. However, the subaltern counter-narratives from indigenous populations concerning their health and rights can more accurately shape our understanding of the severity of these health effects caused by the mining industry.

It has been shown that the chemical nor-binaltorphimine (norBNI) inactivates the k-opioid receptor (KOR) for days or weeks in vivo, and also results in downstream activation of c-Jun N-terminal kinase (JNK). If JNK is blocked in vivo by SP610025 or by knocking out the JNK1 gene, norBNI inactivates the KOR for just hours. We hypothesize that there is a JNK mediated inactivation of the KOR. To further investigate this, I will create constructs that will allow us to study the interactions of JNK and arrestin, two proteins that are involved in the transduction of KOR signaling. I aim to express a version of each JNK1 splice variant tagged with luciferase and arrestin3 tagged with Venus in human embryonic kidney (HEK293) cells that have been transfected with rat KOR. Venus and luciferase are two bioluminescent molecules that emit different wavelengths of light depending on their distance from one another. After treating these cells with norBNI, I can dtermine the level of their interaction by using a bioluminescence resonance energy transfer (BRET) assay. These experiments will provide useful data in determining the extent of the interactions between JNK1 and arrestin3. This data will help us understand the protein cascade that results in the deactivation of the KOR, and if certain drugs activate certain splice variants. Understanding this pathway has implications in creating analgesics with smaller potential for abuse and addiction, and creating pharmaceuticals that could help with stress related depression and addiction relapse.

Serpentinization, a geological process that occurs in many settings on Earth, generally involves the reaction of olivine, a mineral component of ultramafic, basaltic rocks, with water. The products of serpentinization reactions, including serpentine, carbonates, talc, and saponite, are mineralogical signatures which indicate geochemical environments and processes that are hospitable to microbial life. On Earth, these processes have been shown to sustain dense microbial communities. The identification of serpentine in particular uniquely indicates that serpentinization reactions have occurred. Therefore, the identification of the mineral serpentine on Mars has significant astrobiological implications. Previous studies have identified serpentine in three geologic settings on Mars using near-infrared spectral data collected by the Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) on board the Mars Reconnaissance Orbiter. Minerals are identified by their unique absorptions at specific wavelengths through the examination of CRISM surface reflectance data across 438 wavelengths from 1.0-3.9 microns. Unfortunately, spectral datasets like CRISM contain large volumes of data and each spectrum usually covers an area that contains several unique compositions, or spectral endmembers. Here, we have adapted and applied factor analysis techniques to CRISM data to confirm the previous detections of serpentine and search for new identifications in regions containing olivine-rich basalts. Our methods, factor analysis and target transformation, are more sensitive to subtle, characteristic absorptions as in the spectrum of serpentine than traditional analysis techniques and are a set of methodologies that allow for rapid and automated analysis of large spectral datasets. These methods can be used globally to both identify the number of individual components and test for the presence of and isolate individual endmembers from mixed spectral data. Ultimately, by applying this technique to the global CRISM dataset, we will have a more detailed understanding of aqueous processes like serpentinization on Mars.

This project examines the role of gender inequality in HIV/AIDS and how this inequality perpetuates the susceptibility of women to contract the virus. Focusing on Ghana and Brazil, I explore how culture and society shape an imbalance that potentially increases risks to women in becoming sick through analysis of the relationship between social determinants of health, risk factors and vulnerability. I argue that preventative measures intended to fight against HIV/AIDS have failed to consider the social circumstances that place women at greater risk than men. In my analysis, I draw on and interpret Michel Foucault’s concept of biopolitics using feministic critique and gendered perspectives. I reference anecdotes from Stephanie Nolen’s “28: Stories of AIDS in Africa” to present personalized perspectives of the painfully multifaceted reality of the HIV/AIDS outbreak, while Silvana Paternostro’s personal accounts of sexual culture in Brazil in “In the Land of God and Man: Confronting Sexual Culture in Latin America,” serve as a framework for my examination of gendered social constructs in Brazil. I examine gender relations and the dynamic of marital relationships that often disproportionately allow the male greater power over the female, and how a male dominant ideology is reinforced by politics, culture and social norm. Distinguishing between gender and sex, I look at how gender inequality, the inequality of opportunities and power that follow being a certain sex, may expose mothers, daughters, sisters, and other women at risk in ways we may not have imagined.

Recent evidence has indicated that immune activation in mucosal surfaces is a critical determinant of HIV infection risk. HVTN914 Trial aims to quantitate immune activation in foreskin, blood and rectosigmoid mucosa from HIV-negative, uncircumcised men who have sex with men and who are at high risk for HIV acquisition. RNA of high quality and quantity must be isolated to quantitate rare immune transcripts present in these tissues. I isolated RNA from human foreskin, colon, rectum and blood samples to evaluate the RNA yield and quality. Results indicated that 91% of the samples met the required quality and quantity standards for future tests and could therefore be assessed by Reverse Transcription Polymerase Chain Reaction (RT-PCR). I then amplified an endogenous control mRNA to determine if expression of an endogenous control would be different among isolations. The blood samples amplified twice as rapidly as the other tissue types, indicating normalization to the endogenous control would be required. Our data suggests one possible cause for differential amplification is the increased phenol residue in the tissue isolations compared to blood, which can reduce reverse transcription or polymerase activity.

This project examines the ethical and social frames which facilitate environmental conditions that fuel the rampage of tuberculosis in India. Three key components of the basic biomedical outbreak narrative that are often overlooked will be brought forth to attention: the tuberculosis outbreak as more than just a bacterial spread, social constructs which shape the bacteria’s accessibility to victims, and socioeconomic determinants of treatment (and lack thereof). India accounts for approximately one-fifth of global tuberculosis occurrences, and with over 2 million cases in the country, tuberculosis is a continuously growing health concern, posing a major threat to the well-being of the population. Due to India's developing health care system, pollution, and poverty, these conditions amplify the ease of transmissibility and rate of infection. As treatment regimens are abandoned due to social stigma, medical side effects, and overwhelming price tags, the bacterium which causes TB (mycobacterium tuberculosis), tends to mutate and become drug-resistant to varying degrees; increasing the difficulty of containment and scientific development of effective medicines. Furthermore, the capitalist structure of the pharmaceutical industry contributes to the financial challenges which prevent the eradication of tuberculosis. Drawing on the theory of biopolitics while using sources of statistical information and analytical discourse,  my presentation seeks to explore various properties of the disease and discover how the complex interconnectedness of these underlying issues attribute to forms of structural violence, perpetuating the growth of tuberculosis in India.

The aim of this study was to further the understanding of mechanisms that underlie the pruritic and antipruritic effects of kappa opioid receptor drugs. The kappa opioid receptor (KOR) has been studied for its potential therapeutic benefits. It has been previously shown that the kappa opioid receptor antagonist, 5’-guanidinonaltrindole (GNTI), causes compulsive, hind-leg scratching in mice, while the kappa opioid receptor agonist, nalfurafine, inhibits this pruritic effect. There are applications for this antipruritic effect in medicine, to counteract symptoms in kidney-failure patients. By learning more about the mechanisms of this anti-pruritic effect, better drugs can be developed in the future with increased specificity and decreased side effects. We pre-treated male C57/BL6 mice with either saline or nalfurafine (0.05mg/kg, s.c. flank), and challenged them 20 minutes later with 5’-GNTI (0.03mg/kg, s.c. behind the neck) or saline. The number of hind-leg scratches in the 30 minutes following GNTI injection were counted as the measure of itch. We compared the results between kappa opioid receptor (KOR) knock-out mice, mu opioid receptor (MOR) knock-out mice, and wild-type mice, to determine the role of KOR and MOR in GNTI-induced itch and nalfurafine inhibition of itch. We concluded that while the pruritic effects of 5’-GNTI are not mediated by KOR or MOR, the antipruritic effects of nalfurafine was KOR dependent.