Routine ingestion of, and contact with, myriad chemical compounds in our environment impacts all aspects of human health, from psychiatric to oncological to acute and chronic toxicities. However, health outcomes resulting from exposure events are strongly influenced by genetic variability present in genes underlying pertinent metabolic pathways. There are four genes that have been identified in the literature as playing crucial roles in multiple and interrelated biochemical pathways, and which may have broad implications for human health. These genes include DRD4, which codes for the dopamine D4 receptor and has been linked to numerous neurological conditions including addictions, schizophrenia, bipolar disorder, and Parkinson’s disease; 5-HTTLPR, the serotonin-transporter-linked region that codes for the serotonin receptor and may be involved in a host of behaviors including affective disorders and addictions; CYP2E1, a member of the cytochrome P450 family involved with the metabolism of many endogenous and exogenous chemicals including ethanol; and GSTT1, which is involved with the metabolism of toxic compounds including many carcinogens. In order to offer an accessible advanced preventative health screening tool to patients addressing numerous potential conditions, we attempted to implement a multiplexed polymerase chain reaction that would allow us to simultaneously assay these four genetic variants. We were able to combine the GSTT1 and CYP2E1 assays into a single reaction, however we were unable to find compatible reaction conditions for 5-HTTLPR and DRD4 either together or with GSTT1 and CYP2E1.