Kaposi's Sarcoma-Associated Herpesvirus (KSHV) is a gamma2-herpesvirus that infects humans and can cause tumors in immunosuppressed individuals. Rhesus rhadinovirus (RRV) is a related herpesvirus of macaques that is similar to KSHV, but has significant differences as well. While KSHV infections are predominantly latent with only low levels of spontaneous replication, RRV replicates much more readily. For this reason, RRV is a useful tool in studying the mechanisms of reactivation and replication of rhadinoviruses. We sought to identify the regulatory promoter elements of the replication and transactivator (Rta) gene, which induces the lytic stage of the virus. To find this, we transfected cells with different length clones of the Rta promoter region and tested the activity using luciferase assays. We identified a potential binding site of the transcription factor Sp1 and tested its function through mutagenesis. Our results indicate that the Sp1 binding site is required for high level promoter activity suggesting that this is an important regulatory element within the promoter sequence. We also tested the activity of the RRV Rta promoter in the presence of the RRV latency associated nuclear antigen (LANA), a gene known to inhibit replication through suppression of Rta expression in KSHV. We found that with LANA present, the activity of the Rta promoter decreases significantly. Because Rta has a critical role in viral replication, the regulation of the Rta promoter by the cellular factor Sp1 and the viral gene LANA may have important implication on the RRV life cycle.