Chronic social-stress is known to lead to a number of aberrant behaviors, including anxiety and depression. Using a mouse model of social-defeat stress, this project aims to better understand the relationship between the kappa-opioid receptor (KOR), a G-protein coupled receptor (GPCR) previously shown to be activated by multiple forms of stress, and its role in generating anxiety-like and aversion behaviors in mice. Different forms of stress lead to the release of the endogenous KOR agonist, dynorphin, which activates an intracellular signal transduction cascade resulting in anxiety-like and aversion behaviors. Prior studies have demonstrated that the long-acting KOR antagonist, norbinaltorphimine (norBNI), is able to reduce social-defeat induced social avoidance behaviors when administered prior to social defeat stress exposure. Although these studies have implicated the KOR system in social-defeat induced behaviors, pretreatment of pharmacotherapies prior to a stress event is limited in therapeutic benefit.  In this research, the ability of norBNI to reverse repeated social-defeat induced changes in social behaviors was tested by administering norBNI following exposure to the social-defeat stress. The results have so far shown that post treatment with norBNI is able to effectively counter the altered social interaction behaviors caused by a repeated social-defeat stress; moreover, the reversal of the stress-induced social interaction deficits also correlates with a reduction in anxiety-like behaviors. These data suggest that social interaction deficits are due to increased anxiety in previously benign social settings, and norBNI has the therapeutic potential to reverse these social-stress induced behavior alterations.

The purpose of this study is to determine how contemporary American society and its military view the concept of dying and how one is able to have “a good death.” For primary sources, I use Medal of Honor citations, letters home, newspaper obituaries and articles. Preliminary results have shown that the best way one could die was in the action of saving another’s life. Medal of Honor citations alone have indicated this. Of the 258 Medals of Honor, which have been awarded, often posthumously, since the start of the Vietnam War, 56% have been awarded for actions that demonstrated self-sacrifice for others. In the conflicts after Vietnam the proportion of Medal of Honor citations in regard to self-sacrifice have risen: of the 12 Medals of Honor that have been awarded, self-sacrifice made up 75% of the citations. I expect to find more corroborating evidence as I continue my research. It is hoped that information gleaned from this research will help mental health professionals better treat veterans by assuaging any potential survivor’s guilt that military personnel may possess.

Many species have been driven, or nearly driven, to extinction by overexploitation. Without a proper understanding of their complexity, we will continue disrupting ecosystems with unsustainable harvesting. For example, the concept of maximum sustainable yield places priority on maximizing harvests by ignoring most of the consequences. Resource populations are reduced to stimulate productivity without fully accounting for the effects of doing so. To what extent we can sustainably reduce resource populations. Empirical patterns in the reduction of prey populations by natural predators fully account for ecosystem complexity to inform us. We analyzed data from numerous published studies in which the population size of various prey species (marine, fresh-water and terrestrial) had been monitored before and after a predator species was either introduced or removed. The population size was compared with and without predators, to determine the extent to which resource populations are reduced by nonhuman predators. The effects of natural predation is a variable that is currently being ignored as a source of guidance in regard to population reduction, and hope that this work can contribute to more holistic decision-making in this regard. The overall objective is that of maintaining sustainable populations, ecosystems, and fisheries.

The year of 1863 proved to be a turning point in the American Civil War, one reason being the faith of Abraham Lincoln and his spiritually based conviction that the young nation of America could no longer ignore the question of slavery. Lincoln's personal beliefs had a definite effect on his presidency as he struggled over why God did not seem to take a hand in either side of the war. Battles were won and lost on both sides, frustrating everyone who thought the war would be short and decisive. Lincoln, feeling that God had instructed him to do so, enacted the Emancipation Proclamation. This ended up changing the tide of the war and showed how powerful the element of faith can be in the life of a president. As his motivation changed from simply wanting to keep the southern states in the union to actually insisting that slavery had to end, the Emancipation Proclamation and the subsequent victory at Gettysburg finally made Lincoln feel as if Providence was taking a hand.

Ultra-high vacuum (UHV) techniques make it possible to observe interactions on clean, ordered surfaces without the complexity of realistic systems.  Final construction and testing of an improved UHV adsorption microcalorimeter capable of measuring the heat of adsorption of metals on single crystalline surfaces have recently been completed.  This calorimeter is the first of its kind to employ an electron beam evaporator to create a high, stable flux of metal atoms with low source radiation, which is then pulsed onto the thin single crystalline sample. Due to the properties of the electron beam evaporator, metals with high heats of sublimation such as Pd, Pt, and Au can be evaporated while maintaining the base pressures necessary for maintaining ideal surface conditions.  The new chamber is also equipped with a sample preparation chamber in which metal oxide surfaces can be grown on single crystalline samples.  Absolute calibration of the microcalorimeter was achieved by measuring the heat of formation of Cu multilayers on a Pt(111) surface, indicating that the theory behind our electron beam evaporator is correct, and will allow for the first direct measurements of the heats of adsorption of catalytically important metals such as Pt, Pd, and Au on any surface.

The reduction of CO₂ has become a global priority as the concerns of climate change and the adverse effects of atmospheric carbon dioxide on the environment continue to rise. One method of reducing CO₂ emissions is through conversion and utilization as a chemical feedstock. CO₂ is cheap, nontoxic, and can be recycled to produce valuable new products, making it an attractive feedstock. The increasing importance of CO₂ utilization processes requires a better understanding of the underlying mechanisms of its conversion. The industrial conversion of CO₂ is typically achieved using a heterogeneous catalyst, usually consisting of small transition metal particles supported on a high surface area metal oxide. Our project focuses on elucidating the mechanism of the reverse water-gas shift (RWGS) reaction on platinum, an extensively studied catalyst with a highly active surface. Our goals are (1) to quantitatively measure the RWGS rate under conditions of low H coverage and (2) to compare the measured rate to the rate of CO₂ dissociation (the rate-limiting step according to the surface redox mechanism) and to the theoretical rate calculated using a micro-kinetic model based on the associative mechanism. The information we gather from the investigation will clarify whether the RWGS reaction proceeds on platinum via the surface redox mechanism or the associative mechanism.

In the last decade, Seattle, like many other cities across the world, has tried to encourage bicycling by developing new bicycle infrastructures, cultures, and policies. In many cities, these efforts often start nearly from ‘scratch’. However, in The Netherlands, bicycling has been an important part of the transportation network for more than 100 years, with comprehensive infrastructure in place from policies developed during the 1960’s and 70’s. Our team, composed of two members, spent several months in Groningen, The Netherlands, on an exchange where we collected data on the bicycle infrastructure success that this region of the world is renowned for. Working with the Multimodal Project Development team at Seattle Department of Transportation (SDOT), we brought the lessons-learned and design of the Netherlands to our home, Seattle. We have evaluated the design of four critical Seattle intersections: 43rd Ave NE and University Way, 7th Ave NE and NE 40th St., 25th Ave NE and the Burke Gilman Trail, and 34th Ave N and N Fremont Ave. and compared them to successful infrastructure in Groningen. Through background research, interviews, and observation, we have assessed the comfort, safety, and directness of these selected intersections using quantitative and qualitative measures. Based on this data, we have suggested two alternatives for each intersection: one based on Dutch design which is novel to Seattle and very ambitious, and one of which is more practical and less expensive. The possible results of this study are far-reaching and significant. Whether our designs are adopted in some form by SDOT or not, our final designs have influenced SDOT employees. We are taking a step towards making our community a safer, healthier, and more enjoyable place for all street users.

My research examines fairness and equity in the distribution of three wastewater treatment types across seven counties in the Puget Sound region. I begin my analysis with a thorough exploration into the concepts of fairness and equity, within the context of a variety of disciplines. Drawing on this analysis, I then look into the costs of these different systems, making sure to take into account all applicable considerations, including implementation, maintenance, and repair costs. Specifically, I investigate the costs of combined-sewer systems, separated sewer systems, and onsite septic systems at the household scale. Lastly, I use Geographical Information Systems and spatial analysis to compare the costs of these systems to their spatial distribution through the subject counties, and to the household median income within their respective census tracts. This methodology allows me to uncover any inequities in the way that the costs of these treatment types are distributed, and address a specific instance of fairness within urban planning that has yet to be investigated.